วันอังคารที่ 25 กุมภาพันธ์ พ.ศ. 2563

Research article Protective effect of polysaccharide-protein complex from a polypore mushroom, Phellinus rimosus against radiation-induced oxidative stress

Jini Joseph, Sudheesh Narayana Panicker, Kainoor Krishnankutty Janardhanan

Department of Microbiology, Amala Cancer Research Centre, Thrissur, Kerala, India

Objectives: Ionizing radiation induces severe oxidative stress in the body resulting an imbalance in prooxidant and antioxidant status in the cell. The aim of the present study is to investigate the protective effect of polysaccharide protein complex (PPC-Pr) isolated from the mushroom Phellinus rimosus against the oxidative stress induced by gamma radiation. Methodology: PPC-Pr complex was isolated from the aqueous extracts of P. rimosus. The complex was administered to Swiss albino mice at a concentration of 5 and 10 mg/kg body weight intraperitoneally for 5 days consecutively and exposed to 4 Gy of gamma irradiation. Animals were sacrificed 1 day after irradiation and the antioxidant parameters such as glutathione, glutathione peroxidase, superoxide dismutase, catalase, glutathione reductase as well as lipid peroxidation were evaluated in both liver and brain tissues to evaluate oxidative stress. Amifostine, a standard radioprotective agent, was used as a positive control. In vitro DNA damage was assessed using the comet assay. Survival studies were also carried out to determine the protective role of PPC-Pr against radiation-induced delayed oxidative stress. Results: PPC-Pr treatment enhanced the declined levels of antioxidants and comet parameters to a significant level, indicating its antioxidant as well as DNA protecting potential. Significant increase in the survival rate of animals was also observed in irradiated animals treated with PPC-Pr complex. The results were comparable to the standard drug amifostine. Discussion: The results indicate profound effects of PPC-Pr against radiation-induced oxidative stress. The findings suggest potential therapeutic use of PPC-Pr in radiotherapy.

Keywords: Radioprotection, Antioxidants, Oxidative stress, Polysaccharides, Phellinus rimosus, Immunomodulation, Mushrooms, Irradiation

https://www.tandfonline.com/doi/pdf/10.1179/1351000211Y.0000000018

Polysaccharide protein complex isolated from mushroom Phellinus rimosus (berk.) Pilat alleviates γ radiation-induced toxicity in mice.

Joseph J,

Smina TP,

Janardhanan KK

Cancer Biotherapy & Radiopharmaceuticals, 28 Jun 2011, 26(3):299-308
DOI: 10.1089/cbr.2010.0905 PMID: 21711114

Abstract

Ionizing radiations generate reactive oxygen species in irradiated tissue that induces several pathophysiological changes in the body. Radiotherapy induced toxicity is a major dose-limiting factor in anticancer treatments. Radioprotective agents are of significant importance in medical, industrial, environmental, military, and space applications. Radioprotective effect of polysaccharide protein complex (PPC-Pr) isolated from mushroom, Phellinus rimosus, was evaluated in Swiss albino mice. PPC-Pr (5 and 10 mg/kg bwt, i.p.) significantly increased leukocyte count, bone marrow cellularity, glutathione content, and activities of antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase in blood as well as intestinal mucosa when compared with the irradiated control group. Histopathological observation of intestinal jejunal mucosa revealed the tissue protective effects of PPC-Pr. Further radioprotective activity of PPC-Pr was in a dose-dependent manner. The findings suggest potential radioprotective efficacy of PPC-Pr.

https://europepmc.org/article/med/21711114

Original Articles

Polysaccharide Protein Complex Isolated From Mushroom Phellinus rimosus (Berk.) Pilat Alleviates γ Radiation-Induced Toxicity in Mice

Jini Joseph

Thozhuthum Parambil Pathmanabhan Smina

, and

Kainoor Krishnankutty Janardhanan

Published Online:5 Jul 2011https://doi.org/10.1089/cbr.2010.0905

Ionizing radiations generate reactive oxygen species in irradiated tissue that induces several pathophysiological changes in the body. Radiotherapy induced toxicity is a major dose-limiting factor in anticancer treatments. Radioprotective agents are of significant importance in medical, industrial, environmental, military, and space applications. Radioprotective effect of polysaccharide protein complex (PPC-Pr) isolated from mushroom, Phellinus rimosus, was evaluated in Swiss albino mice. PPC-Pr (5 and 10 mg/kg bwt, i.p.) significantly increased leukocyte count, bone marrow cellularity, glutathione content, and activities of antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase in blood as well as intestinal mucosa when compared with the irradiated control group. Histopathological observation of intestinal jejunal mucosa revealed the tissue protective effects of PPC-Pr. Further radioprotective activity of PPC-Pr was in a dose-dependent manner. The findings suggest potential radioprotective efficacy of PPC-Pr.

https://www.liebertpub.com/doi/10.1089/cbr.2010.0905

Phellinus rimosus against radiation-induced oxidative stress.

Protective effect of polysaccharide-protein complex from a polypore mushroom, Phellinus rimosus against radiation-induced oxidative stress.

Joseph J1, Panicker SN, Janardhanan KK.

Author information

Abstract

OBJECTIVES:

Ionizing radiation induces severe oxidative stress in the body resulting an imbalance in prooxidant and antioxidant status in the cell. The aim of the present study is to investigate the protective effect of polysaccharide protein complex (PPC-Pr) isolated from the mushroom Phellinus rimosus against the oxidative stress induced by gamma radiation.

METHODOLOGY:

PPC-Pr complex was isolated from the aqueous extracts of P. rimosus. The complex was administered to Swiss albino mice at a concentration of 5 and 10 mg/kg body weight intraperitoneally for 5 days consecutively and exposed to 4 Gy of gamma irradiation. Animals were sacrificed 1 day after irradiation and the antioxidant parameters such as glutathione, glutathione peroxidase, superoxide dismutase, catalase, glutathione reductase as well as lipid peroxidation were evaluated in both liver and brain tissues to evaluate oxidative stress. Amifostine, a standard radioprotective agent, was used as a positive control. In vitro DNA damage was assessed using the comet assay. Survival studies were also carried out to determine the protective role of PPC-Pr against radiation-induced delayed oxidative stress.

RESULTS:

PPC-Pr treatment enhanced the declined levels of antioxidants and comet parameters to a significant level, indicating its antioxidant as well as DNA protecting potential. Significant increase in the survival rate of animals was also observed in irradiated animals treated with PPC-Pr complex. The results were comparable to the standard drug amifostine.

DISCUSSION:

The results indicate profound effects of PPC-Pr against radiation-induced oxidative stress. The findings suggest potential therapeutic use of PPC-Pr in radiotherapy.

PMID:: 22340512
DOI:: 10.1179/1351000211Y.0000000018

[Indexed for MEDLINE]

https://www.ncbi.nlm.nih.gov/pubmed/22340512

Chemopreventive activity of a macrofungus Phellinus rimosus against N-nitrosodiethylamine induced hepatocellular carcinoma in rat.

2006;5(4):309-21.

Chemopreventive activity of a macrofungus Phellinus rimosus against N-nitrosodiethylamine induced hepatocellular carcinoma in rat.

Ajith TA1, Janardhanan KK.

Author information

Abstract

Chemoprevention is an important alternative approach to control cancer. Chemical substances with multiple inhibitory properties would be a welcome addition to the class of chemopreventive drugs. In this study, we investigated the antioxidant, anti-inflammatory, antimutagenic and cancer preventive activities of aqueous extract of a macrofungus Phellinus rimosus (Berk) Pilat. The extract exhibited superoxide anion (O2-), hydroxyl radical (*OH), nitric oxide (NO*) scavenging and lipid peroxidation inhibiting activities. The inhibitory concentrations required by the extract to scavenge 50% (IC50) of the superoxide anion, hydroxyl radical and nitric oxide generated were 126 +/- 5.1, 71 +/- 4.7 and 31 +/- 4.5 microg/ml respectively. The concentration required to inhibit 50% of Fe2+ induced lipid peroxidation in rat liver homogenate was 318 +/- 2.4 microg/ml. The extract showed significant (P<0.05) anti-inflammatory activity in a dose dependent manner. Extract (100 mg/kg body wt, p.o) inhibited 44.5, 45.4 and 47% carrageenen, dextran and formalin induced inflammations respectively. The antimutagenic activity was determined by the Ames' Salmonella mutagenecity assay using histidine mutant Salmonella typhimurium strains. The extract at concentration of 5 mg/plate showed antimutagenecity against benzo[a]pyrene (B[a]P) and 4-nitro-o-pheneylenediamine (NPDA) induced mutations of TA98 and TA100 respectively. Anticarcinogenic activity was evaluated using N-nitrosodiethylamine (NDEA) induced hepatocellular carcinoma (HCC) in rats. Serum gamma glutamyl transpeptidase (GGT), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and alkaline phosphatase (ALP) activities and lipid peroxidation level (MDA) were elevated significantly (P<0.05) in the NDEA alone treated group of animals. Treatment of the extract (25 and 50 mg/kg body wt, p.o.) prior to the NDEA administration decreased the serum GGT, GOT, GPT and ALP activities and MDA level in a dose dependent manner. The NDEA alone treated animals showed altered serum albumin/globulin ratio (A:G ratio), hyperfibrinogenaemia, increased hepatic glutathione S-transferase (GST) activity, glutathione-peroxidsae (GPx) activity and reduced glutathione (GSH) level compared to the extract plus NDEA treated group. The extract also inhibited in vitro aniline hydroxylase (AH) activity of rat liver induced by phenobarbitone in a dose dependent manner. The results, thus suggest the significant chemopreventive properties of the aqueous extract of the Phellinus rimosus against NDEA induced hepatocellular carcinoma by its antioxidant, anti-inflammatory and antimutagenic activities.

PMID:: 17024971

[Indexed for MEDLINE]

https://www.ncbi.nlm.nih.gov/pubmed/17024971

Antimicrobial, cytotoxic and antioxidant activity of selected basidiomycetes from Yemen.

2005 Oct;60(10):776-80.

Antimicrobial, cytotoxic and antioxidant activity of selected basidiomycetes from Yemen.

Al-Fatimi M1, Wurster M, Kreisel H, Lindequist U.

Author information

Abstract

Dichloromethane, methanol and aqueous extracts of 23 selected Basidiomycetes species fruiting bodies collected in Yemen were screened in vitro for their antibacterial activities against three Gram-positive bacteria (Staphyloccocus aureus, Bacillus subtilis, Micrococcus flavus), two Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and against one yeast fungus (Candida maltosa), as well as for their cytotoxic and antioxidant activity. The highest antibacterial activity was shown by extracts from Agaricus sp. (Type 1), Coriolopsis caperata, Ganoderma colossus, Ganoderma resinaceum, Phellorinia herculea and Tulostoma obesum. Strong antioxidative effects employing the DPPH assay were exhibited by methanol extracts from Ganoderma resinaceum, Inonotus ochroporus, Phellinus rimosus and Phellorinia herculea. The results provide evidence that some of the studied fungi might be potential sources for new biologically active agents.

PMID:: 16259127

[Indexed for MEDLINE]

https://www.ncbi.nlm.nih.gov/pubmed/16259127

Phellinus igniarius

สมุนไพรนี้ เราจัดส่งได้ทั่วโลก โดยคุณเพิ่มค่าจัดส่งอีกเพียงเล็กน้อย ซื่งมันเล็กน้อยมากสำหรับคุณ ตัวอย่างเช่นค่าจัดส่ง ที่น้ำหนักรวมแพคกิ้ง ไม่เกิน 1.5 กกไปประเทศอังกฤษ แบบ อีเอ็มเอส เพียง 2200บาท แบบธรรมดาเพียง 1300 บาท หรือแบบลงทะเบียนเพียง 1200 บาท เท่านั้นเอง (โดยยังไม่รวมภาษี ณ.ประเทศของคุณ ถ้าอาจจะมีคุณต้องชำระเอง)เมื่อรวมกับราคาสินค้า อีก 1500 บาท จึงเป็นจำนวนเงินที่คุณจ่ายเพียงเล็กน้อย เมื่อเทียบกับ สุขภาพที่ดีของคุณ เราหวังว่าจะพบกับคุณได้เร็วๆนี้

This product can be shipped worldwide by paying some more postage. For example, a postage rate for an 1.5 kg EMS parcel shipped to UK is 2,200 baht, a normal parcel is 1,300 baht, and a registered parcel is 1,200 baht (an additional taxes in your country not included). The cost of the product is 1,500 baht. It is a reasonable price because this product can bring you good health. We look forward to seeing you soon.

วันพฤหัสบดีที่ 13 กุมภาพันธ์ พ.ศ. 2563

จัดส่งวันที่ 13-2-63

น้ำใบมะละกอสด มีงานวิจัยที่บอกว่ารักษามะเร็งได้จริงไหม หรือถ้าเอาใบสดหรือแห้งมาดองเหล้า จะใช้ทดแทนกันได้ไหม แล้ะสารตัวไหนในมะละกอ ที่อ้างว่ารักษามะเร็งได้

น้ำใบมะละกอสด มีงานวิจัยที่บอกว่ารักษามะเร็งได้จริงไหม หรือถ้าเอาใบสดหรือแห้งมาดองเหล้า จะใช้ทดแทนกันได้ไหม แล้ะสารตัวไหนในมะละกอ ที่อ้างว่ารักษามะเร็งได้
.
ตอบ ยังไม่พบรายงานวิจัยของน้ำใบมะละกอสดที่มีฤทธิ์รักษามะเร็ง แต่มีงานวิจัยพบว่า สารสกัดน้ำ สารสกัดปิโตรเลียมอีเทอร์ สารซาโปนิน และสาร carpaine ที่สกัดจากใบ มีฤทธิ์ต้านการเจริญเติบโตของเซลล์มะเร็งหลายชนิด เช่น มะเร็งเต้านม (MCF-7), มะเร็งตับ (HepG2), มะร็งปอด (PC14), มะเร็งเม็ดเลือดขาว (lymphoid leukemia L1210, lympholytic leukemia P388), มะเร็งตับอ่อน เป็นต้น ซึ่งเป็นเพียงการทดลองในหลอดทดลอง ยังไม่พบการศึกษาในคน

https://doi.org/10.1016/0277-5379(86)90341-X

Daphnane diterpene esters have previously been shown to have antineoplastic activity in vivo against the growth of P-388 lymphocytic leukemia cells. These studies demonstrate cytotoxic activity of genkwadaphnin and gnidilatidin against P-388 lymphocytic leukemia, L-1210 lymphoid leukemia and human KB carcinoma cell growth in vitro. At the ed50 values in the respective tumor lines DNA synthesis was preferentially suppressed in all three cell lines. RNA synthesis was essentially unaffected by the agents. Protein synthesis inhibition by the two agents demonstrated selectively, e.g. in P-388 cells significant inhibition, in L-1210 cells marginal inhibition and in KB cells no inhibition was observed at these concentrations. Multiple sites in DNA synthesis were found to be inhibited by the daphnane diterpene esters. Two to three times the ed50 concentration in the respective tumor lines was required to observe suppression of DNA synthesis. Purine de novo synthesis appeared to be the major site of inhibition, with inosine monophosphate dehydrogenase and phoshoribosyl pyrophosphate amido transferase activities being inhibited in all three tissue lines. Dihydrofolate reductase activity was inhibited, significant only in the P-388 and KB cells. The magnitude of the enzyme suppression by the agents varied with the tumor line. However, the degree of enzyme suppression was of sufficient magnitude to account for the observed purine and DNA synthesis inhibition by the daphnane diterpene esters.